The kit uses a technique termed multicolor probe melting curve analysis, which allows for multiple mutations to be identified in a single reaction tube on a real-time PCR instrument. By using the combinations of different melting temperature curves generated via differently fluorophore-labeled probes, each mutation can be identified through a post-PCR melting curve analysis. Of the 9 different mutations on the spike protein (N501Y, A570D, D614G, P681H, T716I, S982A, D1118H, Y144del and HV69-70), four mutations (N501Y, D614G, HV69-70 and Y144del) were used as the detection targets. When these four mutations simultaneously occur, a B.1.1.7 strain can be confirmed as proven by a bioinformatics study.
The kit comes with “For research only” label until CE labeling is complete and can be applied to SLAN and BioRad cyclers.
Availability per January 17, place your orders now.